1 Scope
This part of ISO 14240 specifies a method for the estimation of microbial biomass
of soils by measurement of total extractable organic biomass material mainly from
freshly killed microorganisms. The method is also applicable to the estimation of
microbial nitrogen and microbial ninhydrin-reactive nitrogen in soil, but this part
of ISO 14240 describes only the measurement of extractable organic carbon. The fumigation-extraction
(FE) method is applicable to aerobic and anaerobic (water-logged, paddy) soils over
the whole range of soil pH. Biomass can be also measured in soils containing actively
decomposing substrates and soils supersaturated with potassium sulfate solution.
NOTE Chloroform fumigation also affects soil fauna. The contribution of carbon from these
organisms is generally small (< 5 %) and can usually be neglected.