1 Scope
This document specifies a fluorimetric method for the determination of alkaline phosphatase
(ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk,
skimmed milk and flavoured milks.
This method is applicable to milk and milk-based drinks from cows, sheep and goats.
It is also applicable to milk powder after reconstitution.
The instrument used for the determination of ALP can read activities up to 7 000 milliunits
per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free
milk so as to obtain a measurement not higher than 7 000 mU/l.