National foreword

This British Standard is the UK implementation of ISO 21702:2019.

The UK participation in its preparation was entrusted to Technical Committee PRI/21, Testing of plastics.

A list of organizations represented on this committee can be obtained on request to its secretary.

This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.

Compliance with a British Standard cannot confer immunity from legal obligations.

This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2019.

Foreword

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The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).

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This document was prepared by Technical Committee ISO/TC 61, Plastics, Subcommittee SC 6, Ageing, chemical and environmental resistance.

Any feedback or questions on this document should be directed to the user’s national standards body. A complete listing of these bodies can be found at www.iso.org/members.html.

Introduction

Antibacterial-treated porous and non-porous products have been widely accepted and used among general consumers as their new choices to purchase for the additional function, which are different from what traditional materials had in terms of material protection.

Recently, antiviral-treated porous and non-porous products have been also in the market.

The measuring test method of antibacterial activity on non-porous products is described in ISO 22196.

The measuring test method of antibacterial activity on porous products (textiles) is described in ISO 20743.

The measuring test method of antiviral activity on porous products (textiles) is described in ISO 18184.

This document is the test method of antiviral activity on non-porous products. It is written based on ISO 22196 and ISO 18184.

In ISO 22196, the scope has been expanded to include surfaces made of plastics and other non-porous materials, thus this document is intended to be applicable to products such as plastics, coating materials, ceramics, natural and artificial leathers, stainless, rubbers, etc.

1 Scope

This document specifies proper methods for measuring antiviral activity on plastics and other non-porous surfaces of antiviral-treated products against specified viruses. Due to the individual sensitivities, the results of one test virus might not be applicable for other viruses.

2 Normative references

3 Terms and definitions

4 Materials

4.1 Virus and host cell to be used for the tests

4.2 Reagents

4.3 Culture medium and solutions

5 Apparatus

5.1

5.2

5.3

5.4

5.5

5.6

5.7

5.8

5.9

5.10

5.11

5.12

5.13

5.14

5.15

5.16

5.17

5.18

5.19

5.20

5.21

5.22

5.23

5.24

5.25

6 Preparation

6.1 Restoration of host cell from cryopreservation

6.2 Subculture of host cell

6.3 Cell culture for measuring virus infectivity titer

6.4 Preparation of test inoculums

6.5 Preparation of test specimens

6.6 Control test

7 Test procedure

7.1 Preparation of test specimen

7.2 Inoculation of test specimens

7.3 Contact of the inoculated test specimens

7.4 Recovery of virus from test specimens

7.5 Determining the infectivity titer of virus by plaque assay

8 Expression of results

8.1 Determination of the infectivity titer of virus

8.2 Conditions for a valid test

8.3 Calculation of the antiviral activity

8.4 Effectiveness of the antiviral agent

9 Repeatability and reproducibility

10 Test report

Bibliography

BS ISO 21702:2019

Measurement of antiviral activity on plastics and other non-porous surfaces

Current

Published:

31 May 2019