Foreword

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This document was prepared by Technical Committee ISO/TC 216, Footwear.

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1 Scope

This document specifies a test method for evaluating the antimicrobial (either antibacterial or antifungal, or both) activity of footwear and footwear components.

This document is applicable to all types of footwear and footwear components employing diffusing antimicrobial treatments.

2 Normative references

3 Terms and definitions

4 Principle

5 Safety

6 Apparatus

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9

6.10

6.11

6.12

6.13

6.14

6.15

7 Reagents and culture media

7.1 General

7.2 Water

7.3 Nutrient broth (NB)

7.4 Nutrient agar (NA)

7.5 Tryptic soy broth (TSB)

7.6 Tryptone soy agar (TSA)

7.7 Malt medium

7.8 Malt extract agar (MEA)

7.9 Physiological saline (sodium chloride solution)

7.10 Buffer solution

8 Test microorganisms

9 Preparation of specimens

9.1 General

9.2 Test and control specimens

9.3 Pretreatment of specimens

10 Test procedure

11 Expression of results

11.1 Judgement of test effectiveness

11.2 Calculation of zone of inhibition

12 Test report

Bibliography