1 Scope
This document describes a method using isotopically labelled standards for the quantitative
determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone
(ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry
(MS/MS).
A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and
OTA in food intended for infants and young children (e.g. infant cereals, milk-based
powders), in spices, in dried fruits and in nuts.
The method has been validated through an intercollaborative study on different commodity
groups: cereals and cereal-based products including food for infant and young children,
nuts, spices, dried fruits and milk powder. The measuring range of each mycotoxin
in these naturally contaminated and/or spiked food samples were:
| — |
AFB1: |
0,085 7 µg/kg – 11,4 µg/kg; |
| — |
AFB2: |
0,079 2 µg/kg – 12,5 µg/kg; |
| — |
AFG1: |
0,062 8 µg/kg – 20,9 µg/kg; |
| — |
AFG2: |
0,052 0 µg/kg – 15,0 µg/kg; |
| — |
AFM1: |
0,034 2 µg/kg – 0,110 µg/kg; |
| — |
OTA: |
0,448 µg/kg – 17,2 µg/kg; |
| — |
DON: |
45,2 µg/kg – 743 µg/kg; |
| — |
ZEN: |
9,57 µg/kg – 131 µg/kg; |
| — |
T-2: |
10,3 µg/kg – 57,9 µg/kg; |
| — |
HT-2: |
9,50 µg/kg – 81,8 µg/kg; |
| — |
FB1: |
31,1 µg/kg – 4 260 µg/kg; |
| — |
FB2: |
44,2 µg/kg – 1 300 µg/kg. |
The measuring ranges of the method for each mycotoxin/matrix combination are given
in Table 8.