BS EN 14526:2017

What is BS EN 14526-HPLC method for the determination of saxitoxin-group toxins in shellfish about?

BS EN 14526 discusses the determination of saxitoxin-group toxins in shellfish. BS EN 14526 is a European standard that provides the HPLC method for the determination of saxitoxin-group toxins in shellfish to ensure it is safe for consumption.

BS EN 14526 specifies a method for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; the sum of isomers), gonyautoxin 2 and 3 (GTX2,3; the sum of isomers), gonyautoxin 5 (GTX5 also called B1), gonyautoxin 6 (GTX6 also called B2), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; the sum of isomers), N-sulfocarbamoyl-gonyautoxin 1 and 2 (C1,2; the sum of isomers) and (depending on the availability of certified reference materials (CRMs)) N-sulfocarbamoyl-gonyautoxin 3 and 4 (C3,4; the sum of isomers) in (raw) mussels, oysters, scallops and clams.

Laboratory experience has shown that the method under BS EN 14526 also applies in other shellfish and cooked shellfish products.

The method described in BS EN 14526 was validated in an interlaboratory study and was also verified in a EURL-performance test aiming at the total toxicity of the samples.

Who is BS EN 14526-HPLC method for the determination of saxitoxin-group toxins in shellfish for?

BS EN 14526 on HPLC method for the determination of saxitoxin-group toxins in shellfish is useful for:

• Food scientists
• Food technologists
• Food laboratories
• Food health and safety departments

Why should you use BS EN 14526-HPLC method for the determination of saxitoxin-group toxins in shellfish?

Paralytic shellfish poisoning (PSP) toxins are derivatives of saxitoxin. These toxins have been detected in filter-feeding bivalve molluscs in various parts of the world. Paralytic shellfish poisoning is characterized by symptoms varying from a slight tingling sensation or numbness around the lips to fatal respiratory paralysis.

BS EN 14526 provides you with apparatus, methods, procedures, HJPLC determination, identification of PSP toxins, toxin calculation, precision, and much more.

The methods and procedures provided by BS EN 14526 assist in improving the quality of shellfish and reducing the risk to human health due to consumption of saxitoxin.

By adopting BS EN 14526 the user can improve the quality of the shellfish and thus, assist in reducing health risks.

 What is changed since the last update?

BS EN 14526:2017 supersedes EN 14526:2004, which is withdrawn. BS EN 14526:2017 has made some technical changes compared to the previous edition EN 14526:2004. These include:

• The applicability is greater as more samples were tested in interlaboratory studies
• The extraction procedure in 6.2 has been revised.
• The chromatographic conditions in Clause 7 have been revised
• Guidelines for calculation in presence of several toxins were introduced
• The method has been additionally validated in several interlaboratory studies, and the precision data in Annex A have been revised