BS EN 15452 is a European standard that serves technical specifications for the determination of iron chelated in fertilizers, ensuring the ready availability of iron to plant for optimal plant growth in agricultural applications.
BS EN 15452 specifies a method for the chromatographic determination of the amount of iron chelated by each of the individual isomers of the chelating agent ortho-para EDDHA (o,p-EDDHA) in fertilizers.
BS EN 15452 method allows the identification of this chelating agent and the determination of the water-soluble fraction of iron chelated by this chelating agent.
Note 1: BS EN 15452 method is also suitable for the determination of the amount of iron chelated by each of the individual isomers of the chelating agent ortho-ortho EDDHA (o,o-EDDHA) in fertilizers.
Note 2: o,o-EDDHA and o,p-EDDHA are abbreviations used in BS EN 15452 for the sake of simplicity. Complete names are given in Annex C.
Note 3: The substances o,o-EDDHA and o,p-EDDHA both exist as different stereoisomers. For o,o-EDDHA a meso form and a d/l pair (the racemic isomers) exist, for o,p-EDDHA two different d/l pairs exist. All four stereoisomers are observed separately in BS EN 15452.
Note 4: Currently, an analytically pure standard only exists for o,o-EDDHA. The method for o,p-EDDHA has been developed with an o,p-EDDHA standard containing an uncertain concentration of o,p-EDDHA.
Note 5: BS EN 15452 method is not applicable for the determination of the amount of free chelating agent.
BS EN 15452 on the determination of iron chelated in fertilizers applies to:
Even in alkaline soil, the chelates act as a carrier, allowing the iron to be absorbed by the plant's roots. Chelated iron can be used as a supplement to treat and prevent iron deficiency in plants that do not produce chelates naturally.
BS EN 15452 provides users guidance on the HPLC method for the determination of iron chelated in fertilizers. BS EN 15452 also provides users with a step-by-step procedure for the method, enabling users to do the method correctly.
BS EN 15452 provides users guidance on principle, interferences, reagents, apparatus, sampling, sample preparation, procedure, expression of results, precision, and test report, enabling the accurate detection of iron chelated in fertilizers.
With obedience and compliance to BS EN 15452, users can develop and assess fertilizers to determine the iron chelated content, enabling the prevention of iron deficiency in plants.
BS EN 15452:2008 supersedes DD CEN/TS 15452:2006, which is now withdrawn.
EN 15452:2008