NOTE 1 The method specified in this International Standard is also applicable to the determination of nicotine in cigarette smoke condensates obtained by non‑standard smoking.

NOTE 2 In countries not in a position to use the gas‑chromatographic method, reference should be made to ISO 3400 for the determination of total nicotine alkaloids. In such cases, values obtained using the method described in ISO 3400 may be used with the addition of a note in the expression of results.