This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:
sampling strategy and process, including type, storage, transportation, extraction, quality;
nucleic acid library preparation
design and review process including sequencing strategy and assessment;
database construction;
bioinformatics analysis and report
validation and verification for bioinformatics pipeline, and database
This document applies to laboratories and research organizations.