1 Scope
This document specifies a method for the determination of individual intact glucosinolates
in feed materials including oilseeds and oilseed products and in compound feeds by
high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry
(MS/MS).
The method specified in this document has been successfully validated by collaborative
trial in the following matrices: rape seed, camelina seed, Brassica oleracea seeds, mixed oilseeds, rape seed flakes, compound feed for bovine, porcine and poultry.
The method is applicable for the quantitative determination of epiprogroitrin, glucoalyssin,
glucoarabin, glucobrassicanapin, glucobrassicin, glucocamelinin, glucoerucin, glucoiberin,
gluconapin, gluconapoleiferin, gluconasturtiin, glucoraphanin, glucoraphenin, glucotropaeolin,
homoglucocamelinin, 4-hydroxyglucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin,
progoitrin, sinalbin and sinigrin.
The concentration ranges tested in the collaborative trial for each individual glucosinolate
and for the total glucosinolate content are summarized in Table 1.
Table 1
—
Summary of glucosinolate concentration ranges tested in the collaborative trial
| Glucosinolate
|
Number of samples with acceptable results
|
Tested concentration range
mmol/kg
|
|
Min
|
|
Max
|
| Epiprogoitrin |
7 |
0,01 |
|
0,93 |
| Glucoalyssin |
6 |
0,02 |
|
2,10 |
| Glucoarabin |
3 |
0,31 |
|
6,15 |
| Glucobrassicanapin |
5 |
0,01 |
|
0,38 |
| Glucobrassicin |
5 |
0,02 |
|
0,31 |
| Glucocamelinin |
3 |
0,82 |
|
16,1 |
| Glucoerucin |
3 |
1,07 |
|
15,6 |
| Glucoiberin |
3 |
1,51 |
|
18,5 |
| Gluconapin |
6 |
0,23 |
|
1,68 |
| Gluconapoleiferin |
5 |
0,01 |
|
0,33 |
| Gluconasturtiin |
7 |
0,01 |
|
11,0 |
| Glucoraphanin |
5 |
0,01 |
|
3,11 |
| Glucoraphenin |
1 |
|
15,6 |
|
| Glucotropaeolin |
2 |
0,03 |
|
18,3 |
| Homoglucocamelinin |
3 |
0,17 |
|
3,23 |
| 4-Hydroxyglucobrassicin |
6 |
0,23 |
|
7,33 |
| 4-Methoxyglucobrassicin |
1 |
|
0,16 |
|
| Neoglucobrassicin |
5 |
0,01 |
|
0,13 |
| Progoitrin |
6 |
0,62 |
|
14,8 |
| Sinalbin |
4 |
0,01 |
|
41,1 |
| Sinigrin |
3 |
0,25 |
|
23,7 |
| Total content |
8 |
1,48 |
|
117,3 |