BS EN ISO 12966-4:2015

What is ISO 12966-4-Determination of fatty acid methyl esters (FAMEs) in animal and vegetable fats and oils about?  

ISO 12966 is an international standard that discusses the determination of FAMEs in animal and vegetable fats and oils for determining the total fat content in foods. 

ISO 12966-4 is the fourth part of a multi-part series that specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC).  

Fatty acid methyl esters from C8 to C24 can be separated using ISO 12966-4 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.  

The method under ISO 12966-4 is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources. 

Note1: The method under ISO 12966-4 is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of ISO 12966-4. 

Note2: ISO 12966-4 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils. 

Who is ISO 12966-4-Determination of FAMEs in animal and vegetable fats and oils for? 

ISO 12966-4 on the determination of FAMEs in animal and vegetable fats and oils is useful for: 

• Food scientists  
• Food technologists  
• Food laboratories  
• Food health and safety departments 

Why should you use ISO 12966-4-Determination of FAMEs in animal and vegetable fats and oils? 

Fatty acid methyl esters (FAME) are a type of fatty acid ester that are derived by the transesterification of fats with methanol. The molecules in biodiesel are primarily FAME, usually obtained from vegetable oils by transesterification. They are used to produce detergents and biodiesel  

ISO 12966-4 provides you guidance with the apparatus, sampling, preparation of the test, preparation of methyl esters from fats, oils, and fatty acids, capillary gas chromatography method and procedure. 

The methods and procedures provided by ISO 12966-4 assist in detecting FAMEs in animal and vegetable fats and oils and ensures improving the quality of detergents and biodiesel. 

With obedience and compliance to ISO 12966-4, you can enhance the quality of the animal and vegetable fats and oils and manufacture a standardized high-quality product.