1 Scope
This International Standard gives general guidelines for the enumeration of coliforms. It is applicable to
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— products intended for human consumption and for the feeding of animals, and
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— environmental samples in the area of food production and food handling,
by means of the technique of counting colonies after incubation on a solid medium
at 30 ◦C or at 37 ◦C.
NOTE The temperature is subject to agreement between the parties concerned. In the case
of milk and milk products, the temperature of incubation is 30 ◦C.
This technique is recommended when the number of colonies sought is expected to be
more than 100 per millilitre or per gram of the test sample.